Analysis Note

ControlMouse 3T3 fibroblasts Skin (Basement Membrane).

Application

Anti-Integrin α5 Antibody, C-terminus, Intracellular detects level of Integrin α5 & has been published & validated for use in ELISA, IC, IF, IH, IP, RIA & WB.

Western Blot Analysis:
1:1000 dilution of a previous lot was used.

ELISA:
1:500-1:2000 dilution of a previous lot was used in ELISA.

Immunoprecipitation:
Recommended use of 5 µL of antibody for 5x106 cells.

Immunohistochemistry:
1:1000 dilution of a previous lot was used in tissue staining; suggested for use on acetone fixed tissue only.

Immunocytochemistry:
1:1000 of a previous lot was used in immunocytochemistry.

Optimal working dilutions must be determined by the end user.

General description

Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. The alpha-3 subunit exists in two different splice variants, denoted as "A" and "B". The only difference that results from this differential splicing is a total change in the cytoplasmic domain, while the extracellular domain stays the same. Knock-out mice lacking this subunit show prenatal lethality and abnormalities in the kidneys.

Immunogen

Synthetic peptide derived from the C-terminal sequence (cytoplasmic domain) of the human alpha5 integrin subunit.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Rabbit polyclonal antiserum in buffer containing 0.05% sodium azide.

Quality

Routinely evaluated by Western Blot on HUVEC lysates.

Western Blot Analysis: 1:1000 dilution of this lot detected integrin alpha 5 on 10 µg of HUVEC lysates.

Specificity

Antibody recognizes integrin alpha5. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine labeled endothelial cells. No cross-reactivity to alpha1, alpha2, alpha3, alpha4, alpha6 or alphaV. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and it is thus, intracellular.

Reactivity with other species has not been determined.

Target description

114 kDa